C-terminal hemagglutinin tag
WebAug 26, 2024 · The overexpressed GFP-tagged NT proteins were produced at an elevated level relative to the endogenous full-length protein (hemagglutinin (HA)-tagged) (Figure 1B,C). Truncation of the first 11 amino acids (NT12) appeared to slightly increase the steady state level of the expressed protein (albeit, not statistically significant), but further ... WebThe MBP tag can be fused at the N- or C-terminus of the protein and can be easily cleaved using 3C protease. Fluorescent protein reporters Large polypeptide sequences from this …
C-terminal hemagglutinin tag
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WebThe mature HA1/HA2 complex contains a membrane-proximal stalk domain containing the N- and C-terminal portions of HA1 and the entirety of HA2 and a membrane-distal receptor-binding domain containing receptor-binding and vestigial esterase subdomains (Figure 3B). Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human influenza virus. The HA-tag is derived from the HA-molecule corresponding to amino acids 98-106. HA-tag has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins … See more The DNA sequences for the HA-tag include: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The resulting amino acid sequence: YPYDVPDYA (Tyr … See more • Biology portal • Protein tag • SpyTag See more • Field J, Nikawa J, Broek D, MacDonald B, Rodgers L, Wilson IA, Lerner RA, Wigler M (May 1988). "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method". Molecular and Cellular Biology. 8 … See more
WebRecombinant Influenza A Virus Hemagglutinin H3 protein (His tag) is a HEK 293 Protein fragment 17 to 529 aa range, ... His tag C-Terminus. Additional sequence information. Influenza A Virus Hemagglutinin H3 trimeric (A/Beijing/32/92) (H3N2) protein (Genebank #: … WebThe RPL22HA C-Terminal Hemagglutinin Tag Can Be Used To Immuno-precipitate Polysomes. Fig. 2 is a cartoon that outlines the Rib-oTag methodology. Crossing the …
WebATCC hemagglutinin ha epitope tag Hemagglutinin Ha Epitope Tag, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more WebIdentification of the C- Terminal Residue. The C-terminal residue is determined by the use of either a chemical reagent or the enzyme carboxypeptidase. The chemical reagent …
WebInfluenza hemagglutinin HA (ΔTM) (H3N2) (A/Perth/16/2009) (Genebank # ACS71642). A trimerization domain sequence has been introduced into the C-terminal of HA to stabilize the formation of trimer HA. Associated products Specifications Preparation and Storage General Info Images Protocols Datasheets and documents References (0)
WebC-terminal hemagglutinin tag) (21). Cultures were preincubated with STI571 or the -secretase inhibitor L-685,458 for 2 h, then pulse labeled for 30 min with [35S]methionine [35S]cysteine Easy Tag Protein Labeling Mix (Perkin–Elmer) and chased for 2 h with inhibitors present. Media were immunoprecipitated with pear and mayo and cheese recipeWebApr 8, 2024 · C-terminal SUMO amides have been previously shown to inhibit SUMOylation cascades . ... The human histone eH3.1 (C96A/C110A) gene with a C-terminal FLAG and hemagglutinin tag was subcloned … pear and mint ciderWebApr 14, 2024 · We investigated how CO4 affects the OsCERK1–OsCEBiP interaction and found that CO4 treatment reduced the coimmunoprecipitation of OsCERK1-HA (OsCERK1 fused to a C-terminal Hemagglutinin tag) with OsCEBiP-FLAG (OsCEBiP fused to a C-terminal DYKDDDDK peptide tag) in Arabidopsis protoplasts ( Fig. 3 A ). pear and olive reviews