WebJan 24, 2024 · Furthermore, HCT116 are not addicted to mtKRAS for survival, minimizing selection pressure to acquire compensatory mutations when mtKRAS dosage is reduced 17. To attain a representative coverage of the EGFR PPIN we selected 95 bait proteins (Supplementary Data 1 ) based on a highly curated EGFR signaling network map 18 and a … WebDisease relevance of HCT116 Cells. In cell growth studies, 500 and 1,000 microM Lev increased the toxicity of 5-FU in HCT 116 cells in an additive fashion . ... cell cloning of …
MSH3 expression does not influence the sensitivity of colon …
HCT116 cells have a mutation in codon 13 of the KRAS proto-oncogene, and are suitable transfection targets for gene therapy research. The cells have an epithelial morphology and can metastasize in xenograft models. When transducted with viral vectors carrying the p53 gene, HCT116 cells remain arrested in the G1 phase. The proliferation of HCT116 colonies was found to be inhibited by 5-Fu/P85 copolymer micelles. Furthermore, it was found that the knockout of MARCH2 limited … WebAug 3, 2015 · SW480 and HCT116 colorectal cancer cells have KRAS G12V and G13D mutations, respectively ( 23 ). These KRAS-mutated cell lines are relatively resistant to neratinib (IC 50 values of 430 nmol/L) compared with the KRAS WT cell lines, paralleling the results obtained with IMCE–KRAS cells (Supplementary Figs. S1B and S3). diseases of the hypothalamus gland
Isogenic HCT116 cell lines with heterozygous or homozygous …
WebHCT 116 cell line was isolated from the colon of an adult male with colon cancer. It has a mutation in codon 13 of the ras proto-oncogene and can be used as a positive control for … WebCCLE Name: HCT116_LARGE_INTESTINE. COSMIC ID: 905936. Sanger ID: 1920. Cell Line Passport: SIDM00783. Cellosaurus RRID: CVCL_0291. Growth Pattern: Adherent. ... WebJul 6, 2024 · Although mutation analysis was restricted to a panel of 612 genes, the high mutation load in MSI/POLE mutated cell lines allowed for detection of expected mutation signatures. In the cell lines with a lower mutation load, broader sequencing coverage (whole exome or genome sequencing) would be more appropriate for accurate analyses of … diseases of silkworm slideshare ppt