WebMay 13, 2024 · Reads Mapped Confidently to Intronic Regions Reads Mapped Confidently to Exonic Regions Reads Mapped Confidently to Transcriptome Reads Mapped Antisense to Gene Fraction Reads in Cells Total Genes Detected Median UMI Counts per Cell 1-MAP2 2-MAP2 3-MAP2 4-MAP2 5-MAP2 6-MAP2 7-MAP2 8-MAP2 1-AT8 2-AT8 3-AT8 4-AT8 5 …
General status reporting: Single sample analysis
WebReads Mapped Confidently to Exonic Regions: Fraction of reads that mapped to the exonic regions of the genome with a high mapping quality score as reported by the aligner. Reads Mapped Confidently to Intronic Regions: Fraction of reads that mapped to the intronic regions of the genome with a high mapping quality score as reported by the aligner ... WebReads Mapped Confidently to Intergenic Regions Reads Mapped Confidently to Intronic Regions Reads Mapped Confidently to Exonic Regions Reads Mapped Confidently to Transcriptome Reads Mapped Antisense to Gene Fraction Reads in Spots Total Genes Detected Median UMI Counts per Spot; 3,673: 223,859: 2,610: 822,237,691: 97.1%: 96.1%: … popular now on us
Why do I have a high percentage of reads mapping to intronic regions
WebJan 11, 2024 · 2 Answers. You could use BEDOPS bedmap to map reads to introns, using 1) the --count operator to do counting of reads overlapping by your criteria; and, 2) the --indicator operator to do a true/false operation, where reads are contained entirely within the intron. For instance, to count reads that overlap introns by at least 25 bases, use ... WebMay 17, 2024 · The method uses reads from not only exonic 102 but also intronic and intergenic regions (Figure 1 and Supplementary Dr. Disco technical 103 specification). These split and spanning reads are converted and inserted into a breakpoint graph 7. The 104 graph is analysed to find reads originating from the same junctions. WebReads mapped confidently to intronic regions - 比对到内含子区域. Reads mapped confidently to exonic regions - 比对到外显子区域. Reads mapped confidently to transcriptome - 比对到转录组的reads，这些读数可以用来UMI的计数. Reads mapped antisense to gene - 比对到基因的相反的reads. 4. 细胞数目评估 ... shark raw helmet custom