WebMar 18, 2024 · As an alternative to biotinylation, the newly exposed protein thiol groups in hydroxylamine-treated cell lysates can also be directly captured by sulfhydryl-reactive solid phase carriers, such as thiopropyl Sepharose beads (Acyl-RAC assay) (Forrester et al. 2011; Kumar et al. 2016) or magnetic microspheres (Zhang et al. 2024). WebThiopropyl Sepharose 6B Thiopropyl Sepharose™ 6B contains reactive 2-thiopyridyl disulphide groups attached to Sepharose through a chemically stable ether linkage, see …
Activation of ErbB3–PI3-kinase pathway is correlated with …
WebDec 14, 2000 · Plasmin products were collected on lysine–Sepharose beads 2 and either separated on ... pH 7.4 buffer and batch adsorbed for 60 min at 20 °C to 6 ml of swollen thiopropyl-Sepharose ... WebSepharose is a bead-formed agarose-based gel filtration matrix. Sepharose is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose 2B, Sepharose 4B and … how rhythm and blues became rock and roll
Proteomic Profile of Reversible Protein Oxidation Using PROP
WebMar 1, 1987 · Reaction with Thiopropyl-Sepharose 6B yielded hexynyl-nortestosterone-Sepharose beads with a ligand density of about 7 micromoles/ml beads. One-hundred μl of these beads adsorbed 71% of the PgR present in 1 ml ofcytosol from T47D cells. This adsorption was inhibited by 10 μM progesterone but not Cortisol, indicating the specificity … http://wolfson.huji.ac.il/purification/PDF/Thiophilic_Chromatography/Thiopropyl_Sepharose.pdf WebNov 21, 2016 · The resuspended samples were then loaded to 80 μl thiopropyl Sepharose beads (GE Healthcare) in the presence of 40 mM sodium ascorbate and rotated in the dark for 1.5 h at room temperature and ... merriam webster dictionary founders